Browsing by Author "Falco Restrepo, Aura Dayana"
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Item Actividad antimicrobiana de Fitocannabinoides como principios activos en plantas medicinales: una revisión sistemática(Universidad Santiago de Cali, 2024) Rivera Vargas, María Yuliel; Suarez Osorio, Liseth; Falco Restrepo, Aura DayanaPhytochemical compounds have long been an alternative to traditional medicine, which is why the industrial sector is dedicated to the manufacture of products such as drugs, cosmetics and veterinary products, and have considered innovating their lines from raw materials derived from the extraction of active compounds from plants. In this way, plant material with various attributes or substances that are allowed and regulated by government entities for use and marketing has been sought. Due to the above, it was proposed to carry out this systematic review that aims to identify medicinal plants that contain phytocannabinoids and that have antimicrobial activity. The consulted databases corresponding to 83, of which documents dating from the years 2010 to 2022 were found, however, 24 were discarded, since they were related to clinical aspects of veterinary use and others due to the years of publication, the which are outside the range of the minimum 10 years to be taken into account for the development of the systemic reviewItem “Aislamiento de bacteriófagos provenientes de una muestra de origen ambiental con capacidad de infectar salmonella spp”(Universidad Santiago de Cali, 2020) Álvarez Pérez, Juan Martín; Falco Restrepo, Aura DayanaSalmonellosis is a Foodborne Disease (FDB) that occurs due to contamination by bacteria of the Salmonella genus in edibles such as chicken and pork, as well as in products from poultry farms. Because the indiscriminate use of antibiotics in chickens has contributed to the selection of resistant bacteria, it is necessary to implement natural alternatives that allow the control of bacterial populations in poultry farms, bacteriophages being a possible option due to their high specificity to infect bacteria. Using the double layer method, bacteriophages were found in an environmental sample of chicken manure from a poultry farm located in the municipality of Palmira, Valle del Cauca, capable of infecting a strain of Salmonella enterica subsp. enterica ATCC 14028.Item Determinación de los cambios que genera la presencia de los Iones Mg2+, Ca2+, y Na+en la proliferación de salmonelófagos líticos(Universidad Santiago de Cali, 2024) Guazá Balanta, María José; Aranaga Arias, Carlos Andrés; Falco Restrepo, Aura DayanaOne of the most common foodborne diseases worldwide is salmonellosis, which is caused by the bacterial genus Salmonella spp. The resistance to antibiotics that this bacteria has conferred begins the search for alternatives for its treatment, phage therapy being one of these. The adhesion of bacteriophages to the cell surface is the first and one of the main stages in viral infection. There are ions such as Mg+2, Ca+2 and Na+ that work as cofactors for the adhesion of bacteriophages to their hosts, resulting in an increase in the viral population. That is why in this work, based on the determination of the viral titer of a bacteriophage infective for Salmonella (4x109 PFU/mL), the changes in its proliferation in the presence of Mg+2, Ca+2 and Na+and ions were determined. The Mg+2 ion was the one in which changes were found that can potentiate the obtaining of the bacteriophage from a complex sample such as a solid environmental matrixItem Molecular characterization of KPC-producing Klebsiella pneumoniae isolated from patients in a Public Hospital in Caracas, Venezuela(Elsevier Doyma, 2017-03-11) Falco Restrepo, Aura Dayana; Velásquez Nieve, Mariel Alexandra; Takiff, HowardIntroduction: Klebsiella pneumoniae carbapenemase (KPC)-producing bacteria are amongst the most important causative agents of nosocomial infections worldwide. Isolates of this bacterium have been identified in Venezuela but little is known about their local spread. The aim of this study was to perform the molecular characterization of KPC-producing strains isolated from 2012 to 2013 in a public hospital in Caracas, Venezuela. Methods: Twenty-two K. pneumoniae clinical isolates phenotypically classified as KPC producing were subjected to PCR screening for the presence of blaKPC genes and their location within transposon Tn4401. The blaKPC PCR product was sequenced to identify the KPC alleles. Genotypic analysis was performed by means of repetitive extragenic palindromic PCR (rep-PCR) and Multi Locus Sequence Typing (MLST). Finally, conjugation and electroporation assays were used to determine whether the blaKPC genes were found in plasmids. Results: All isolates contained the blaKPC-2 variant, and 21 of the 22 were associated with the Tn4401b isoform. The strains were distributed in 8 sequence types (ST), three of which were new. Conjugation and electroporation assays indicated that 95.5% (n = 21/22) of the isolates contained the blaKPC gene in plasmids. Conclusions: This study on circulating bacterial strains and the identification of KPC alleles may help to understand the routes of dissemination and control their spread within this hospital.