Browsing by Author "Fabricio, Mariana F."
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Item The oleaginous yeast Meyerozyma guilliermondii BI281A as a new potential biodiesel feedstock: Selection and lipid production optimization(Frontiers Media S.A., 2017-09-22) Ramírez-Castrillón, Mauricio; Jaramillo-García, Victoria P.; Rosa, Priscila D.; Landell, Melissa Fontes; Vu, Duong; Fabricio, Mariana F.; Ayub, Marco Antônio Záchia; Robert, Vincent A.R.G.; Henriques, João Antonio Pêgas; Valente, PatríciaA high throughput screening (HTS) methodology for evaluation of cellular lipid content based on Nile red fluorescence reads using black background 96-wells test plates and a plate reader equipment allowed the rapid intracellular lipid estimation of strains from a Brazilian phylloplane yeast collection. A new oleaginous yeast, Meyerozyma guilliermondii BI281A, was selected, for which the gravimetric determination of total lipids relative to dry weight was 52.38% for glucose or 34.97% for pure glycerol. The lipid production was optimized obtaining 108 mg/L of neutral lipids using pure glycerol as carbon source, and the strain proved capable of accumulating oil using raw glycerol from a biodiesel refinery. The lipid profile showed monounsaturated fatty acids (MUFA) varying between 56 or 74% in pure or raw glycerol, respectively. M. guilliermondii BI281A bears potential as a new biodiesel feedstock. © 2017 Ramírez-Castrillón, Jaramillo-Garcia, Rosa, Landell, Vu, Fabricio, Ayub, Robert, Henriques and Valente.