Browsing by Author "Espinosa Rosero, Nathaly"
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Item Caracterización molecular y epidemiológica de una cohorte de casos de microfilariasis canina identificados en Cali y municipios cercanos(Universidad Santiago de Cali, 2020) Espinosa Rosero, Nathaly; Rosero López, Ángelo Enrique; Castellanos Sánchez, Angélica María; Vera Lizcano, OmairaFilariae are nematodes that affect different mammals. In canines, the filariae first larval stage is called microfilaria that can be observed circulating in peripheral blood. Dirofilaria immitis is the most prevalent specie worldwide, causing heartworm disease, that can be fatal in canines and affect humans. The objective of this study was the identification of cases of canines with filariasis in Cali (Colombia) and close localities in of one-year period, detected by microscopy, serological methods and the determination of the species by the molecular method PCR–RFLP. In eight cases PCRsequencing was performed. As a result, a total of 102 cases of canine filariasis were detected, of which 82 were molecularly analyzed, finding that 55 (67%) of them corresponded to the Acantacheilonema reconditum specie. The filarial species in the remaining cases could not be molecularly identified, probably due to low parasitaemia by the time of blood sampling. The filariasis cases were statistically associated with males who presented clinical signs of anemia, with low levels of hemoglobin and hematocrit