Browsing by Author "Diazgranados Santos, Maryory Vanessa"
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Item Caracterización molecular de bacterias presentes en la planta de tratamiento de lixiviados del antiguo vertedero de navarro, Santiago de Cali, Valle del Cauca(Universidad Santiago de Cali, 2022) Pachón Soto, Ingrid Alejandra; Diazgranados Santos, Maryory Vanessa; Corrales Ducuara, Alba RocíoThe study of environmental ecology is important to understand the functions, applications and use of bacteria with metabolic capacities to degrade organic compounds; therefore it is important to characterize the bacteria that can be found in various contaminated environments, which through molecular identification more accurate information can be obtained on microbial biodiversity. Therefore, the present study identified cultivable bacteria of environmental importance in six lagoons and a pure leachate that are part of a Leachate Treatment Plant (LTP) located in the Old Navarro Sanitary Landfill (AVN) in the city of Santiago de Cali. Forty six morphotypes were isolated and evaluated from the amplification of the 16S gene (16S rRNA) with the universal primers U1/U2. Sequence analysis was performed using bioinformatics programs and 4 main Phylum Firmicutes, Proteobacteria, Bacteroidete and Actinobateria were recorded, of which Firmicutes was the most abundant. In addition, an association analysis was performed and it was shown that two morphotypes were present in all the sampled ponds: they were identified as Bacillus sp., and Bacillus mycoides. This study represents an advance in the recognition of bacterial biodiversity in the six lagoons that are part of the Treatment Plant (PTL) of the Old Navarro Sanitary Landfill (AVN); Obtaining and generating 27 16S rRNA sequences from bacteria isolated from AVN PTL leachate samples. Because there are few studies on the molecular identification of environmental bacteria in leachate samples, this work constitutes a first report of bacterial species present in the six lagoons and pure leachate that are part of the PTL, contributing to the knowledge of biodiversity. Of cultivable bacteria that are present in the study area for future researchItem Morfotipos bacterianos aislados en el Antiguo Vertedero de Navarro(Editorial Universidad Santiago de Cali, 2020-06) Vergara López, Laura Isabella; Diazgranados Santos, Maryory Vanessa; Ortiz Díaz, Alejandro; Machacado Salas, Maryuris; Rache Arce, Diana Carolina; Rosero García, Doris Amanda