Browsing by Author "De La Cadena, Elsa"
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Item Correction to: Culturable microbial composition in the midgut of Aedes aegypti strains with different susceptibility to dengue-2 virus infection (Symbiosis, (2019), 10.1007/s13199-019-00646-y)(Springer, 2019-11-18) Molina-Henao, Edward H.; Graffe, M. Yineth; De La Cadena, Elsa; Serrato, Idalba M.; Correa, Adriana; Romero, Lizeth V.; Caicedo, Paola A.; Ocampo, Clara B.In the article Culturable microbial composition in the midgut of Aedes aegypti strains with different susceptibility to dengue-2 virus infection (https://usc.elogim.com:2131/10.1007/s13199-019-00646-y) written by Molina-Henao, Graffe, De La Cadena, Serrato, Correa, Romero, Caicedo, and Ocampo, the responses to queries were, unfortunately, omitted by the publisher. One of the authors, Adriana Correa, would like to add the affiliation Universidad Santiago de Cali, Cali, Colombia; and Clara Ocampo should be another corresponding author; her email is claraocampo@cideim. org.co. The Abstract claims that the extrinsic period was ten days when it was 14. In 1. Introduction the publication year of Serrato et al. is given as 2014; actually it is 2017. In the 2.3 Microorganism identification section, the MALDI-TOF matrix solution quantity was 2 µL instead of 2 L; and the year of Marruco et al., 2017, in fact, is 2018. Furthermore, there are spelling errors in Table 1: the name of bacterium isolated from pupae of Cali-MIB (which should be Microbacterium paraoxydans, not Microbacterium paraoxidans), and the database used to identify Acinetobacter bereziniae in pupae from Cali-S was NCBI and not NBI. In the References section, the correct PAST reference is Hammer Ø, Harper DAT, Ryan PD (2001) PAST: paleontological statistics software package for education and data analysis ver 1.89. Palaeontol Electron 4(1):1–9. Moreover, we ask to include the reference Clara B. Ocampo, Paola A. Caicedo, Gloria Jaramillo, Raul Ursic Bedoya, Olga Baron, Idalba M. Serrato, Dawn M. Cooper, Carl Lowenberger, Eric Jan, (2013) Differential Expression of Apoptosis Related Genes in Selected Strains of Aedes aegypti with Different Susceptibilities to Dengue Virus. PLoS ONE 8 (4):e61187. The corrected version is online. © 2019, Springer Nature B.V.Item Detection of carbapenemase-producing Pseudomonas aeruginosa: Evaluation of the carbapenem inactivation method (CIM)(Elsevier Doyma, 2019-03-18) Gutiérrez, Sergio; Correa, Adriana; Hernández Gómez, Cristhian; De La Cadena, Elsa; Pallares, Christian; Villegas, María VirginiaIntroduction: The carbapenem inactivation method (CIM) is a cost-effective assay for detecting carbapenemases. However, its interpretation is unclear for Pseudomonas spp. We evaluate its accuracy when meropenem is changed to imipenem. Methods: We analyzed 266 P. aeruginosa isolates. The CIM method consists of: resuspend bacterial colonies (a full 10 μL loop) in 400 μL water, in which a 10 μg disk of meropenem/imipenem is immersed. After 2 h of incubation (35 °C), remove the disk, place it onto a Mueller-Hinton agar plate previously inoculated with Escherichia coli (ATCC 25922), and incubate at 35 ̊C between 18-24 h. Interpretation criteria (mm of inhibition zone): ≤19 mm, positive; ≥25 mm negative; 20–24 mm, undetermined. Results: Imipenem improves the sensitivity and specificity of CIM when compared to meropenem (99.4% and 98.9%, vs. 91.9% and 94.7%, respectively). Conclusions: The accuracy of CIM for carbapenemase detection in P. aeruginosa is increased with the use of imipenem.