Browsing by Author "Cruz Vega, Yuliana"
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Item Evaluación de la actividad antimicrobiana y anti-biofilm de aceites esenciales de Zingiber officinale, Melaleuca alternifolia, Citrus aurantium dulcis y Eugenia caryophyllat frente a enterobacterias encontradas en el cacao(Universidad Santiago de Cali, 2023) Cruz Vega, Yuliana; Delgado Ospina , Johannes; González, Iván AndrésIn this study, the effect of essential oils of ginger (Zingiber officinale), tea tree (Melaleuca alternifolia), orange (Citrus aurantium dulcis) and clove (Eugenia caryophyllata) on twelve strains of Enterobacteriaceae removed from dried cocoa was investigated. clove and tea tree essential oils were able to inhibit all twelve strains of enterobacteria, at concentrations 1.25 mg/g to 0.625 mg/g and 5.0 mg/g to 2.5 mg/g respectively, while ginger oil alone it was effective against four strains at the highest concentration tested 20 mg/g. On the other hand, orange oil did not show inhibition capacity against any of the evaluated strains. Regarding biofilm formation, the effects of essential oils in Sub-MIC, MIC and Upper-MIC concentrations were evaluated, their action classified according to the percentage of inhibition as stimulating, low, moderate, high and excellent. In biofilm removal, the four essential oils showed significant activity, especially clove and tea tree oils showed better efficacy, preventing biofilm formation between 90% and 100% in enterobacteria, confocal laser scanning microscopy (CLSM) analyzes ) confirmed the results obtained in the inhibition of biofilm formation, especially in the strain Klebsiella pneumoniae spp. pneumoniae B001. In biofilm removal, significant results will be obtained with the use of clove oils, tea tree achieved high and excellent removals, while orange and ginger oil achieved high removals, orange oil presented better removal action. Biofilm compared to ginger oil