Browsing by Author "Correa, Adriana"
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Item Beta-Lactam-Resistant Enterobacterales Isolated from Landfill Leachates(2022-10) Mondragón Quiguanas, Alejandra; Villaquirán Muriel, Miguel Ángel; Rivera, Sandra Patricia; Rosero García, Doris; Aranaga, Carlos; Correa, Adriana; Falco, AuraAntibiotic resistance is one of the main challenges worldwide due to the high morbidity and mortality caused by infections produced by resistant bacteria. In Colombia, this problem has been studied mainly from the clinical perspective; however, it is scarcely studied in the leachates produced in landfills. The objective of this study was to detect, identify and determine the antibiotic sensitivity profile of Enterobacterales isolated from a leachate treatment plant located in Cali, Colombia. Detection was performed using selective culture media, bacterial identification using Matrix-Assisted Laser Desorption/Ionization-Time-Of-Flight (MALDI-TOF, bioMérieux) and by sequencing the gene coding for the 16S ribosomal RNA subunit when discrepancies were observed between phenotypic characteristics and MALDI-TOF. Antibiotic sensitivity profiling was determined using the automated VITEK®2 system (bioMérieux). Twenty-one isolates were obtained, of which Klebsiella pneumoniae was the most frequent (23.8%), and 34% of the isolates showed decreased sensitivity to beta-lactam antibiotics such as cefoxitin, ampicillin/sulbactam and piperacillin/tazobactam. These findings suggest that leachates from landfills could be a reservoir of pathogenic bacteria carrying antibiotic resistance determinants, so periodic microbiological characterization of these effluents should be performed, promoting the One Health approach.Item Comparative In Vitro Activity of Ceftolozane/Tazobactam against Clinical Isolates of Pseudomonas aeruginosa and Enterobacterales from Five Latin American Countries(2022-08) García Betancur, Juan Carlos; De La Cadena, Elsa; Mojica, María F.; Hernández-Gómez, Cristhian; Correa, Adriana; Radice, Marcela A.; Castañeda Méndez, Paulo; Jaime Villalon, Diego A.; Gales, Ana C.; Munita, José M.; Villegas, María VirginiaBackground: Ceftolozane/tazobactam (C/T) is a combination of an antipseudomonal oxyiminoaminothiazolyl cephalosporin with potent in vitro activity against Pseudomonas aeruginosa and tazobactam, a known β-lactamase inhibitor. The aim of this study was to evaluate the activity of C/T against clinical isolates of P. aeruginosa and Enterobacterales collected from five Latin American countries between 2016 and 2017, before its clinical use in Latin America, and to compare it with the activity of other available broad-spectrum antimicrobial agents. Methods: a total of 2760 clinical isolates (508 P. aeruginosa and 2252 Enterobacterales) were consecutively collected from 20 hospitals and susceptibility to C/T and comparator agents was tested and interpreted following the current guidelines. Results: according to the CLSI breakpoints, 68.1% (346/508) of P. aeruginosa and 83.9% (1889/2252) of Enterobacterales isolates were susceptible to C/T. Overall, C/T demonstrated higher in vitro activity than currently available cephalosporins, piperacillin/tazobactam and carbapenems when tested against P. aeruginosa, and its performance in vitro was comparable to fosfomycin. When tested against Enterobacterales, it showed higher activity than cephalosporins and piperacillin/tazobactam, and similar activity to ertapenem. Conclusions: these results show that C/T is an active β-lactam agent against clinical isolates of P. aeruginosa and Enterobacterales.Item Correction to: Culturable microbial composition in the midgut of Aedes aegypti strains with different susceptibility to dengue-2 virus infection (Symbiosis, (2019), 10.1007/s13199-019-00646-y)(Springer, 2019-11-18) Molina-Henao, Edward H.; Graffe, M. Yineth; De La Cadena, Elsa; Serrato, Idalba M.; Correa, Adriana; Romero, Lizeth V.; Caicedo, Paola A.; Ocampo, Clara B.In the article Culturable microbial composition in the midgut of Aedes aegypti strains with different susceptibility to dengue-2 virus infection (https://usc.elogim.com:2131/10.1007/s13199-019-00646-y) written by Molina-Henao, Graffe, De La Cadena, Serrato, Correa, Romero, Caicedo, and Ocampo, the responses to queries were, unfortunately, omitted by the publisher. One of the authors, Adriana Correa, would like to add the affiliation Universidad Santiago de Cali, Cali, Colombia; and Clara Ocampo should be another corresponding author; her email is claraocampo@cideim. org.co. The Abstract claims that the extrinsic period was ten days when it was 14. In 1. Introduction the publication year of Serrato et al. is given as 2014; actually it is 2017. In the 2.3 Microorganism identification section, the MALDI-TOF matrix solution quantity was 2 µL instead of 2 L; and the year of Marruco et al., 2017, in fact, is 2018. Furthermore, there are spelling errors in Table 1: the name of bacterium isolated from pupae of Cali-MIB (which should be Microbacterium paraoxydans, not Microbacterium paraoxidans), and the database used to identify Acinetobacter bereziniae in pupae from Cali-S was NCBI and not NBI. In the References section, the correct PAST reference is Hammer Ø, Harper DAT, Ryan PD (2001) PAST: paleontological statistics software package for education and data analysis ver 1.89. Palaeontol Electron 4(1):1–9. Moreover, we ask to include the reference Clara B. Ocampo, Paola A. Caicedo, Gloria Jaramillo, Raul Ursic Bedoya, Olga Baron, Idalba M. Serrato, Dawn M. Cooper, Carl Lowenberger, Eric Jan, (2013) Differential Expression of Apoptosis Related Genes in Selected Strains of Aedes aegypti with Different Susceptibilities to Dengue Virus. PLoS ONE 8 (4):e61187. The corrected version is online. © 2019, Springer Nature B.V.Item Detection of carbapenemase-producing Pseudomonas aeruginosa: Evaluation of the carbapenem inactivation method (CIM)(Elsevier Doyma, 2019-03-18) Gutiérrez, Sergio; Correa, Adriana; Hernández Gómez, Cristhian; De La Cadena, Elsa; Pallares, Christian; Villegas, María VirginiaIntroduction: The carbapenem inactivation method (CIM) is a cost-effective assay for detecting carbapenemases. However, its interpretation is unclear for Pseudomonas spp. We evaluate its accuracy when meropenem is changed to imipenem. Methods: We analyzed 266 P. aeruginosa isolates. The CIM method consists of: resuspend bacterial colonies (a full 10 μL loop) in 400 μL water, in which a 10 μg disk of meropenem/imipenem is immersed. After 2 h of incubation (35 °C), remove the disk, place it onto a Mueller-Hinton agar plate previously inoculated with Escherichia coli (ATCC 25922), and incubate at 35 ̊C between 18-24 h. Interpretation criteria (mm of inhibition zone): ≤19 mm, positive; ≥25 mm negative; 20–24 mm, undetermined. Results: Imipenem improves the sensitivity and specificity of CIM when compared to meropenem (99.4% and 98.9%, vs. 91.9% and 94.7%, respectively). Conclusions: The accuracy of CIM for carbapenemase detection in P. aeruginosa is increased with the use of imipenem.Item Emerging carbapenem-resistant Aeromonas spp. infections in Cali, Colombia(Elsevier Editora Ltda, 2019-09-18) Rosso, Fernando; Cedano, Jorge Andrés; Parra-Lara, Luis Gabriel; Sanz, Ana María; Toala, Alejandra; Velez, Jolian Fernando; Hormaza, María Paula; Moncada, Pablo Andrés; Correa, AdrianaIntroduction Aeromonas species are renowned enteric pathogens with virulence determinants linked to human diseases, such as gastroenteritis, skin, soft-tissue and muscle infections, and septicemia. A recent concern of resistance in this organism has emerged, especially the presence carbapenemases. Herein we describe a case series of emerging carbapenem-resistant Aeromonas species infection in our hospital in Cali, Colombia. Materials and methods Cases from 2012 to 2018 are reported. Clinical data was abstracted from the clinical charts and laboratory information. Phenotypic detection of resistance was identified using the VITEK®2 system (BioMérieux) and broth microdilution MicroScan WalkAway plus System (Beckman Coulter). CARBA NP-test and multiplex qPCR assay was performed in 11 isolates to identify genes encoding carbapenemases (blaKPC, blaVIM, blaIMP and blaNDM). Results 21 cases of Aeromonas infection in hospitalized patients with phenotypic resistance to carbapenems were studied. The median age was 50 years, 55% (12/21) were male, and 67% (14/21) were healthcare-associated infections (HAI). Aeromonas hydrophila was the most common species (19/21). Forty-three percent (9/21) of the patients were immunocompromised. The mortality was 33% (7/21), and in patients with bacteremia was 100%. Most patients received empirical treatment with meropenem and failed to this treatment. PCR amplification tests showed negative results for the carbapenemases analyzed. Conclusion Emerging phenotypic carbapenem-resistant infection has been seen in our hospital, most as HAI. High mortality was found, especially in immunocompromised patients and in those who failled empirical treatment with carbapenems. As the main carbapenemases tested were negative, carbapenem-resistant could be attributed to an intrinsic metallo-β-lactamase, CphA encoded by the cphA gene, possible hyperproduction of ampC β-lactamase and/or porins expression.Item Identification of Vibrio metschnikovii and Vibrio injensis Isolated from Leachate Ponds: Characterization of Their Antibiotic Resistance and Virulence-Associated Genes(2023-11) Falco, Aura; Villaquirán Muriel, Miguel Ángel; Gallo Pérez, José David; Mondragón Quiguanas, Alejandra; Aranaga, Carlos; Correa, AdrianaThis study aimed to evaluate the antibiotic resistance of 22 environmental Vibrio metschnikovii isolates and 1 Vibrio injensis isolate from landfill leachates in southwestern Colombia. Isolates were identified by Matrix-Assisted Laser Desorption/Ionization–Time-Of-Flight (MALDI-TOF), and 16S ribosomal RNA gene sequencing. Analysis of the susceptibility to six antibacterial agents by the Kirby–Bauer method showed susceptibility of all the isolates to ciprofloxacin and imipenem. We recorded resistance to beta-lactams and aminoglycosides, but no multidrug resistance was observed. The genome of one of the isolates was sequenced to determine the pathogenic potential of V. injensis. Genes associated with virulence were identified, including for flagellar synthesis, biofilm formation, and hemolysins, among others. These results demonstrate that landfill leachates are potential reservoirs of antibiotic-resistant and pathogenic bacteria and highlight the importance of monitoring Vibrio species in different aquatic environments.Item Impact of antimicrobial stewardship programs on antibiotic consumption and antimicrobial resistance in four Colombian healthcare institutions(2022-12) Pallares, Christian; Hernández Gómez, Cristhian; Appel, Tobías Manuel; Escandón, Kevin; Reyes, Sergio; Salcedo, Soraya; Matta, Lorena; Martínez, Ernesto; Cobo, Sara; Mora, Laura; Marín, Adriana; Correa, Adriana; De La Cadena, Elsa; Rodríguez Baño, Jesús; Villegas, María VirginiaBackground Antimicrobial stewardship programs (ASPs) have become a fundamental pillar in optimizing antimicrobial usage, improving patient care, and reducing antimicrobial resistance (AMR). Herein we evaluated the impact of an ASP on antimicrobial consumption and AMR in Colombia. Methods We designed a retrospective observational study and measured trends in antibiotic consumption and AMR before and after the implementation of an ASP using interrupted time series analysis over a 4-year period (24 months before and 24 months after ASP implementation). Results ASPs were implemented according to the available resources in each of the institutions. Before ASP implementation, there was a trend toward an increase in the antibiotic consumption of all measured antimicrobials selected. Afterward, an overall decrease in antibiotic consumption was observed. The use of ertapenem and meropenem decreased in hospital wards, while a decrease in the use of ceftriaxone, cefepime, piperacillin/tazobactam, meropenem, and vancomycin was observed in intensive care units. After ASP implementation, the trend toward an increase of oxacillin-resistant Staphylococcus aureus, ceftriaxone-resistant Escherichia coli, and meropenem-resistant Pseudomonas aeruginosa was reversed. Conclusions In our study, we showed that ASPs are a key strategy in tackling the emerging threat of AMR and have a positive impact on antibiotic consumption and resistance.