Browsing by Author "Carrascal, Edwin"
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Item Efecto de la integración del ADN de papilomavirus humano en carcinoma tracto respiratorio y digestivo superiores(Universidad Santiago de cali, 2012-05-15) Mancilla, Lida Inés; Gheith, Tarik; Carrascal, Edwin; Tamayo, Oscar Mario; García, Felipe; Tommasino, MassimoSe determinó la asociación de la integración de papilomavirus humano (HPV16) con el proceso de carcinogénesis en carcinoma epidermoide de 84 tumores de varias regiones de tracto digestivo superior y sistema respiratorio. El ADN de HPV16 y la expresión de las oncoproteinas E6 y E7 se determinaron con PCR y RT-PCR. Las mutaciones en el gen TP53, utilizando dHPLC y secuenciación. El 27% de los carcinomas evaluados presentó ADN de HPV16. El 37% de los carcinomas presentó el ADN viral integrado al genoma celular y 43% de 54 tumores evaluados expresó ARN de los oncogenes E6 y E7. El gen TP53 presentó mutaciones en el 80% de los tumores evaluados (25). La mayor frecuencia de mutación se observó en el exon 8 de tumores de pulmón y cavidad oral. Se propone la importancia de la integración del ADN de HPV16 en la etiología del carcinoma epidermoide en estas regiones anatómicas.Item Human papillomavirus in upper digestive tract tumors from three countries(2011-12-28) Castillo, Andrés; Koriyama, Chihaya; Higashi, Michiyo; Anwar, Muhammad Maqsood; Bukhari, Mulazim Hussain; Carrascal, Edwin; Mancilla, Lida I.; Matsumoto, Matsutaka; Sugihara, Kazumasa; Natsugoe, Shoji; Eizuru, Yoshito; Akiba, Suminori; Okumura, HiroshiAIM: To clarify human papillomavirus (HPV) involvement in carcinogenesis of the upper digestive tract of virological and pathological analyses. METHODS: The present study examined the presence of HPV in squamous cell carcinomas of the oral cavity (n = 71), and esophagus (n = 166) collected from Japan, Pakistan and Colombia, with different HPV exposure risk and genetic backgrounds. The viral load and physical status of HPV16 and HPV16-E6 variants were examined. Comparison of p53 and p16INK4a expression in HPV-positive and HPV-negative cases was also made. RESULTS: HPV16 was found in 39 (55%) oral carcinomas (OCs) and 24 (14%) esophageal carcinomas (ECs). This site-specific difference in HPV detection between OCs and ECs was statistically significant (P < 0.001). There was a significant difference in the geographical distribution of HPV16-E6 variants. Multiple infections of different HPV types were found in 13 ECs, but multiple infections were not found in OCs. This difference was statistically significant (P = 0.001). The geometric means (95% confidence interval) of HPV16 viral load in OCs and ECs were 0.06 (0.02-0.18) and 0.12 (0.05-0.27) copies per cell, respectively. The expression of p16INK4a proteins was increased by the presence of HPV in ECs (53% and 33% in HPV-positive and -negative ECs, respectively; P = 0.036), and the high-risk type of the HPV genome was not detected in surrounding normal esophageal mucosa of HPV-positive ECs. CONCLUSION: Based on our results, we cannot deny the possibility of HPV16 involvement in the carcinogenesis of the esophagus. © 2011 Baishideng. All rights reserved.Item Role of Human Papillomavirus Type 16 in Squamous Cell Carcinoma of Upper Aerodigestive Tracts in Colombian patients(2011-01-01) Mancilia, Lida Ines; Carrascal, Edwin; Tamayo, Óscar Mario; García, Felipe T.; Vaccarella, Salvatore V.; Gheit, Tarik; Tommasino, MassimoInfections with mucosal High-Risk (HR) Human Papillomavirus (HPV) types are associated with a subset of Squamous Cell Carcinoma (SCC) of Upper Aerodigestive Tract (UAT). However, the prevalence of HR HPV-positive UAT cancers appears to vary in different geographical areas. To evaluate the role of HR HPV infection in Colombian population, we have characterized the prevalence of HPV16 infection and viral transcriptional activity in UAT cancers from 72 Colombian patients including tongue (n = 22), palate (n = 2) and gum (n = 2); larynx (n = 40) and esophagus (n = 6). HPV16 presence was determined by different molecular assays that allowed the evaluation of HPV16 DNA positivity, E6^E7 expression as well as the physical status of the viral genome i.e., integrated versus episomal. HPV16 DNA was detected in 35 and 30.8% of laryngeal and oral cavity cancers, respectively. In contrast, none of the esophageal cancers analyzed were HPV16-positive. Forty one percent of the UAT cancers HPV16 positives harboured the integrated form of HPV16 genome while 18.1% of the cases appeared to have both episomal and integrated viral DNA forms. E6 and E7 expression was detected in 41% of the UAT cancers independently of the HPV16 status. HPV16 DNA integration and E6 and E7 expression did not show any correlation with gender, age and type of cancer. These data provides evidence for the involvement of HPV16 in development of UAT cancers in the Colombian population. © 2011 Academic Journals Inc.