Browsing by Author "Burbano Verdugo, Laura Vanessa"
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Item Caracterización genética y perfil de sensibilidad a metales pesados en aislados de Pseudomonas mendocina provenientes de una Planta de Tratamiento de Lixiviados(Universidad Santiago de Cali, 2022) Burbano Verdugo, Laura Vanessa; Falco, Aura; Aranaga Arias, Carlos AndrésPseudomonas mendocina is a Gram-negative bacillus capable of growing in leachates, which are characterised by high loads of organic matter and contaminants that exert a selective pressure in favour of bacteria that possess antimicrobial resistance determinants, which makes them candidates to evaluate their bioremediation potential. Therefore, in this work, genetic characterisation including genotyping of P. mendocina isolates from a Leachate Treatment Plant was performed through PCR amplification of palindromic extragenic repeat sequences (rep-PCR). In addition, the presence of plasmid DNA and whether it was transferable by conjugation was determined and, finally, the heavy metal sensitivity profile was determined. Some isolates were shown to be genetically related, to carry conjugative plasmids and to grow at different metal concentrations. In conclusion, they are suggested as candidate bioremediators.