Browsing by Author "Betancourt Ayala, Yeiston"
Now showing 1 - 1 of 1
Results Per Page
Sort Options
Item Evaluación antimicrobiana de colistina encapsulada con nanoliposomas recubiertos de quitosano de mediano peso molecular, frente a bacterias gramnegativas sensibles y multidrogoresistentes(Universidad Santiago de Cali, 2021) Betancourt Ayala, Yeiston; Oñate Garzón, Jose Fernando (Director); Rivera Sánchez, Sandra Patricia (Directora)Colistin is a last resort antibiotic which has been reimplemented in clinical practice in order to combat infections by gram-negative and multidrug-resistant bacteria, however, this antibiotic has lost affinity towards this type of bacteria, being caused by the increase and dissemination of strains resistant to this polypeptide. In this work, the antimicrobial activity of colistin encapsulated in nanoliposomes coated with chitosan of medium molecular weight was evaluated against the sensitive Escherichia coli strains, and Pseudomonas aeruginosa sensitive and multidrug resistant strains. Physicochemical properties such as particle size, polydispersity index and zeta potential were determined using Zetasizer nano ZSP. The results for the chitosan-coated systems showed changes in size (from 461.5 nm uncharged to 322.2 nm with colistin), polydispersity index (from 0.219 unloaded to 0.449 with colistin), and zeta potential (from -2.1 mV unloaded). at 15.0 mV with colistin). After encapsulating the colistin, the minimum inhibitory concentration (MIC) of the formulations was determined by the broth microdilution method for P.a ATCC (27853), sensitive population P.a and resistant P.a. For antimicrobial activity, resistance was obtained in strain No. 2, decrease in minimum inhibitory concentration in strain No. 1, sensitivity in strain No. 3 and population strain (W.t).