Browsing by Author "Basante Velasco, Angie Lizeth"
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Item Selección de una cepa nativa de saccharomyces cerevisiae para la producción de etanol a partir de vinaza(Universidad Santiago de Cali, 2022) Basante Velasco, Angie Lizeth; Zúñiga López, Yuli Marcela; Ramírez Castrillón, Mauricio; Zambrano Salgado, Yesid FabiánVinasse comes from the distillation of fermented material in the production of ethanol. Part of the vinasse is recirculated for the fermentation tank decreasing water consumption and production costs, however, in turn it inhibits the growth of yeast. Therefore, the objective of this study was to select a native strain of Saccharomyces cerevisiae tolerant to diluted vinasse for ethanol production purposes. 23 strains of Saccharomyces spp. were evaluated, which were reactivated from the USC mycotheca, including an industrial strain and reference strains as controls. A vinasse medium supplemented with Honey B was designed, determining the concentration of total reducing sugars and volatile acidity, performing fermentations under conditions of oxygen restriction, ambient temperature and constant agitation. Three strains of S. cerevisiae were preselected for their alcohol-producing capacity in synthetic medium, although only the LC010-A strain was evaluated for further experiments. Alcohol concentrations were 6.7% v/v for S. cerevisiae LC010-A and 4.5% v/v for S. cerevisiae NCYC 660 (internal code RNC.1). Yeast strains are usually tolerant to the toxic components of vinasse, when the levels of these acids are less than 3000 ppm approximately, since if this range is exceeded it leads to low yields, and even the inactivation of the yeast. Therefore, these yeasts, especially S. cerevisiae LC010-A, showed tolerance by fermenting substrates containing vinasse and inhibitors, highlighting the potential use of strains native to Valle del Cauca