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Browsing by Author "Balanta Chavarriaga, Leidy Vanessa"

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    Exploración de la presencia de Salmonella spp. en una infusión de cascarilla de cacao
    (Universidad Santiago de Cali, 2021) Balanta Chavarriaga, Leidy Vanessa; Delgado Ospina, Johannes (Director)
    Salmonella is an enteropathogen responsible for foodborne diseases, with the particularity of tolerating high temperatures and low water activity (Aw) when found in food matrices with high lipid content such as cocoa and some of its derivatives. Among the byproducts of cocoa production is the cocoa husk obtained after the processes of fermentation, drying and roasting of the beans, which is used to prepare infusions. It was evaluated the susceptibility of three strains of Salmonella (S. enterica ATCC 10768, S. typhimurium ATCC 14028, S. enterica ATCC 53648) to survive in the conditions typical of cocoa husks destined to the preparation of infusions. The three strains of Salmonella were inoculated in the shell at a concentration of 106 CFU mL-1 and were quantified during the process of elaboration of the infusion. The quantification of Salmonella was developed by the most probable number technique (MPN). It was found that Salmonella did not present any type of survival both in heating and storage. The susceptibility test developed by Kirby Bauer's method suggests that the polyphenols present in cocoa husk inhibit the growth of Salmonella, being S. enterica ATCC 53648 the most susceptible strain to environments with low or high contents of polyphenols.

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