Browsing by Author "Arce Vargas, Nelson Antonio"
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Item Caracterización de la capacidad lignolitica de bacterias presentes en el proceso de compostaje en el ingenio Incauca(Universidad Santiago de Cali, 2025) Arce Vargas, Nelson Antonio; Jaimes Loaiza, Danna Isabella; Fernández Daza, Fabian Felipe; Diaz Caicedo, Lizeth NataliaObjective: To characterize bacterial strains with ligninolytic capacity present in the composting process of The Incauca sugar mill. Methodology: Lignin was extracted from sugarcane bagasse and leaves using the Kraft method. Bacteria were isolated from nine 300 g samples collected from bagasse piles, material in process, and compost. Serial decimal dilutions were plated on MMS agar (minimal salt medium) supplemented with 5 mL of lignin. Colonies with the highest growth were selected, followed by Gram staining and species identification using the VITEK®2 system. Ligninolytic capacity was assessed by UV-Vis spectrophotometry at 260 nm. Results: A total of 18 bacterial isolates were obtained: 4 from bagasse, 6 from material in process, and 7 from compost. Of these, 76.5% were Gram-positive, exhibiting bacillus and coccus morphologies. Five isolates were identified as Enterobacter cloacae complex (n = 3) and Klebsiella pneumoniae (n = 2). The Enterobacter isolates showed higher lignin degradation, with transmittance values ranging from 0.038 to 0.041. Conclusion: Composting serves as a viable source for isolating ligninolytic bacterial species, which are characterized by their ability to colonize organic matter and environmental substrates