Ramírez-Castrillón, MauricioJaramillo-García, Victoria P.Rosa, Priscila D.Landell, Melissa FontesVu, DuongFabricio, Mariana F.Ayub, Marco Antônio ZáchiaRobert, Vincent A.R.G.Henriques, João Antonio PêgasValente, Patrícia2019-07-082019-07-082017-09-221664302Xhttps://repositorio.usc.edu.co/handle/20.500.12421/277A high throughput screening (HTS) methodology for evaluation of cellular lipid content based on Nile red fluorescence reads using black background 96-wells test plates and a plate reader equipment allowed the rapid intracellular lipid estimation of strains from a Brazilian phylloplane yeast collection. A new oleaginous yeast, Meyerozyma guilliermondii BI281A, was selected, for which the gravimetric determination of total lipids relative to dry weight was 52.38% for glucose or 34.97% for pure glycerol. The lipid production was optimized obtaining 108 mg/L of neutral lipids using pure glycerol as carbon source, and the strain proved capable of accumulating oil using raw glycerol from a biodiesel refinery. The lipid profile showed monounsaturated fatty acids (MUFA) varying between 56 or 74% in pure or raw glycerol, respectively. M. guilliermondii BI281A bears potential as a new biodiesel feedstock. © 2017 Ramírez-Castrillón, Jaramillo-Garcia, Rosa, Landell, Vu, Fabricio, Ayub, Robert, Henriques and Valente.enBiodieselFatty acidsLipid productionNile RedRaw glycerolCritical micelle concentrationMeyerozyma guilliermondiiGas chromatographyOptical densityNonhumanLipogenesisArticle