Montoya, AndrésDaza, AlejandroMuñoz, DianaRíos, KarinaTaylor, VivianaCedeño, DavidVélez, Iván D.Echeverri, FernandoRobledoa, Sara M.2019-06-282019-06-282015-09-0100664804https://repositorio.usc.edu.co/handle/20.500.12421/237An evaluation of the leishmanicidal activity in vitro and in vivo of hypericin, an expanded-spectrum photosensitizer found in Hypericum perforatum, is presented. Hypericin was evaluated against intracellular amastigotes in vitro of Leishmania (Viannia) panamensis. A topical formulation containing 0.5% hypericin was developed and assayed in vivo in a hamster model of cutaneous leishmaniasis. Results demonstrate that hypericin induces a significant antiamastigote effect in vitro against L. panamensis by decreasing the number of parasites inside infected cells. The topical formulation of 0.5% hypericin allows healing of L. panamensis-induced lesions upon a topical application of 40 mg/day plus visible-light irradiation (5 J/cm 2 , 15 min), twice a week for 3 weeks. Copyright © 2015, American Society for Microbiology. All Rights Reserved.esAnimalsCell LineCricetinaeHumansLeishmaniaLeishmaniasis, CutaneousPerylenePhotochemotherapyPhotosensitizing AgentsU937 CellsWound Healingdrug absorptiondrug efficacycytotoxicityamphotericin B deoxycholateepidermal growth factorglyceraldehyde 3 phosphate dehydrogenaseArticle